Overview

At VIMM Bioimaging Facility, we provide a broad choice of microscopes for biomedical investigation. The broad array of stereomicroscopes, transmission and fluorescence microscopes, advanced confocal and 2-photon systems allows:

  • fixed, live imaging, in vivo microscopy;
  • cell and tissue, thin and thick slice sample imaging;
  • Fast brightfield and fluorescence imaging;
  • Multidimensional imaging and experiments (multicolor, z-stack, timelapse, spectral, etc);
  • Ratiometric indicator, FRET, FRAP, photo activation experiments;

We offer an experienced microscopy specialist that supports the researcher throughout the process of imaging, from selection and use of the recommended system, to the acquisition of the final image and result. In addition, we provide basic and advanced training of users on each system.

Main Microscopes

LEICA TCS SP5

An inverted laser scanning confocal system, equipped with standard (galvo), and resonant scanner (for high speed imaging) as well as with 3 photomultiplier (PMT) for fluorescence, and one for transmitted light (for bright field and DIC).

Objectives
Leica HC PL FLUOTAR 10x/0.3
Leica HC PL FLUOTAR 20x/0.5
Leica HCX PL Apo lambda blue 40x/1.25 Oil UV
Leica HCX PL Apo lambda blue 63x/1.40 Oil UV

Laser excitation lines:
405nm
458, 476, 488, 494, 514nm Argon
561nm
633nm

Emission Filters: continuously adjustable spectral detector from 400nm to 800nm

Maximum image resolution: 8192×8192 pixels

Zoom: from 1 to 64x

Standard scanner speed: from 1Hz to 1400Hz

Resonant scanner speed: 8000Hz

Acquisition mode
xt (fast line scan)
xz realtime image plane (with z-galvo)
xyt series (timelapse)
xyz series (z-stack)
xy lambda series (spectral image series)
Moreover mix of the above

Application
Live Data Mode
3D reconstruction
Colocalization analysis
Dye Finder, linear unmixing
FRAP, FRET Wizards

ZEISS LSM700

An upright laser scanning confocal system, equipped with 2 PMT for fluorescence and 4 solid state lasers. It is completely motorized, except for XY stage movement.

Objectives
Zeiss N-Achroplan10x/0.25
Zeiss EC Plan-Neofluar 20x/0.5
Zeiss EC Plan-Neofluar 40x/1.3 Oil
Zeiss W Plan-Apochromat 40x/1 Water
Zeiss Plan-Apochromat 63x/1.4 Oil

Solid state laser excitation lines:
405nm
488nm
555nm
639nm

Emission Filter: Selectable filter, plus variable secondary dichroic beam splitter (VSD)
Filters: SP490, SP55, SP640, LP490, LP560, LP640.

Maximum image resolution: 2048×2048 pixels

Zoom: from 0.5x to 40x

Acquisition mode
Multicolor simultaneous or sequential
xt (fast line scan)
xyt series (timelapse)
xyz series (z-stack)
Bleaching or photoactivation

SCIENTIFICA 2 PHOTON

A 2 photon system equipped with a Coherent Chameleon pulsed laser adjastable from 680nm to 1080nm, a galvo scanner, 2 simultaneous high sensitivity GaAsp PMT detectors. The microscope uses a simple interchangeable filter cube system to select and separate the emission light to the PMTs. It is completely motorized, and particularly suitable for in vivo experiment.

Objective
Nikon LWD 16x/0.8 W

Pulsed Laser excitation: from 680nm to 1080nm

Emission filter set:
525-620nm Green Red
480-535nm FRET

Maximum image resolution: 1024×1024 pixels

Zoom: from 1x to 10x

Galvo scanner speed: depending on image resolution, could reach about 30 frames/s

Acquisition mode:
2 colors simultaneous
xt (fast line scan)
xyt series (timelapse)
xyz series (z-stack)
Automated multiple ROI xyzt
Complex experiments

Contact

Denis Bison

Phone: +39 049 7923 291
denis.bison@unipd.it

Fondazione per la ricerca Biomedica Avanzata
Venetian Institute for Molecular Medicine (V.I.M.M.)
via Orus 2
35129 Padova PD
Italy